A simple and inexpensive on-column frit fabrication method for fused-silica capillaries for increased capacity and versatility in LC-MS/MS applications |
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| Authors: | Wang Ling-Chi Okitsu Cindy Yen Kochounian Harold Rodriguez Anthony Hsieh Chih-Lin Zandi Ebrahim |
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| Affiliation: | Department of Molecular Microbiology and Immunology, USC Norris Cell and Tissue Imaging Core, USC/Norris Comprehensive Cancer Center, Keck School of Medicine at USC, Los Angeles, CA, USA. |
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| Abstract: | A modified sol-gel method for a one-step on-column frit preparation for fused-silica capillaries and its utility for peptide separation in LC-MS/MS is described. This method is inexpensive, reproducible, and does not require specialized equipments. Because the frit fabrication process does not damage polyimide coating, the frit-fabricated column can be tightly connected on-line for high pressure LC. These columns can replace any capillary liquid transfer tubing without any specialized connections up-stream of a spray tip column. Therefore multiple columns with different phases can be connected in series for one- or multiple-dimensional chromatography. |
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| Keywords: | Frit fabrication High resolution proteomics Liquid chromatography‐tandem mass spectrometry Multiprotein complex Two‐dimensional chromatography |
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