Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR |
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| Authors: | Slimani Sami Robyns Audrey Jarraud Sophie Molmeret Maëlle Dusserre Eric Mazure Céline Facon Jean Pierre Lina Gérard Etienne Jerome Ginevra Christophe |
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| Institution: | a Hospices Civils de Lyon, Lyon, Franceb Université de Lyon, Centre National de Référence des légionelles, Lyon, Francec INSERM U851, Lyon, Franced Bio-Rad, 3 avenue Raymond Poincaré, 92 430 Marnes la Coquette, France |
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| Abstract: | A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria. |
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| Keywords: | Legionella VBNC Propidium monoazide qPCR |
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