Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia |
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| Authors: | Abraham Sam Chin James Brouwers Huub J M Zhang Ren Chapman Toni A |
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| Affiliation: | a Microbiological Diseases and Diagnostics Research, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, 2568, Australiab School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2500, Australia |
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| Abstract: | Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. |
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| Keywords: | RFLP, Restriction fragment length polymorphism ETEC, Enterotoxigenic E. coli S, Smooth R, Rough LB, Luria Bertani Ont, O-non typable |
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