Natural biopolymer for preservation of microorganisms during sampling and storage |
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Authors: | Sorokulova Iryna Watt James Olsen Eric Globa Ludmila Moore Timothy Barbaree James Vodyanoy Vitaly |
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Affiliation: | a Department of Anatomy, Physiology, and Pharmacology Auburn University, Auburn, AL 36849, United Statesb Clinical Research Facility, 301 Fisher Street, Keesler AFB, MS 39534, United Statesc Department of Biological Sciences, Auburn University, Auburn, AL 36849, United States |
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Abstract: | Stability of microbial cultures during sampling and storage is a vital issue in various fields of medicine, biotechnology, food science, and forensics. We have developed a unique bacterial preservation process involving a non-toxic, water-soluble acacia gum polymer that eliminates the need for refrigerated storage of samples.The main goal of this study is to characterize the efficacy of acacia gum polymer for preservation of pathogenic bacteria (Bacillus anthracis and methicillin-resistant Staphylococcus aureus—MRSA) on different materials, used for swabbing and filtration: cotton, wool, polyester, rayon, charcoal cloth, and Whatman paper.Acacia gum polymer used for preservation of two pathogens has been shown to significantly protect bacteria during dehydration and storage in all tested samples at the range of temperatures (5-45 °C for MRSA and 40-90 °C for B. anthracis). Our results showed higher recovery as well as higher viability during the storage of both bacteria in all materials with acacia gum. Addition of acacia gum polymer to swabbing materials or filters will increase efficacy of sample collection and identification of pathogenic bacteria from locations such as hospitals or the environment. Proposed approach can also be used for long-term storage of culture collections, since acacia gum contributes to viability and stability of bacterial cultures. |
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Keywords: | Bacillus anthracis Methicillin-resistant Staphylococcus aureus Acacia gum Preservation Sampling Storage |
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