High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type"期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type"

Authors:	Douarre Pierre E Cashman William Buckley Jim Coffey Aidan O'Mahony Jim M

Affiliation:	^a Cork Institute of Technology, Cork, Ireland ^b Veterinary Department, Cork County Council, County Hall, Cork, Ireland

Abstract:	In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.

Keywords:	Mycobacterium avium subsp. paratuberculosis Genotyping PCR High resolution melting
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