Capacitive immunosensor for the detection of host cell proteins |
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Authors: | Teeparuksapun Kosin Hedström Martin Kanatharana Proespichaya Thavarungkul Panote Mattiasson Bo |
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Affiliation: | a Department of Biotechnology, Lund University, Box 124, S-22100 Lund, Sweden b Trace Analysis and Biosensor Research Center (TAB), Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand c Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand d Department of Physics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand |
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Abstract: | A new analysis for monitoring host cell proteins in preparations of transgenically produced protein pharmaceuticals is described. A capacitive biosensor with a very high sensitivity is used to monitor trace amounts of host cell proteins. The sensor consists of a gold electrode, the surface of which is well insulated and on which a preparation of a population of polyclonal antibodies raised against the complete protein set-up of the host cell are immobilized.Host cell proteins are present at very low concentrations during the production of a transgenic protein. The system studied here is a model system with an enzyme expressed in Escherichia coli (E. coli). Due to the high sensitivity, it may even be possible to dilute the samples to be analyzed, thereby reducing a negative influence from non-specific binding to the sensor surface. |
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Keywords: | Capacitive immunosensor HCP E. coli Process monitoring |
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