Antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31

Antibodies which specifically recognize the capsid protein (L1) of the human papilloma virus (HPV) serve as an important tool for creation of vaccines against HIV infection. Peptide fragments that corresponded to the 49?C65, 131?C145, 172?C189, 349?C362, and 402?C414 sequences of the L1 protein of HPV type 31 (L1?C31) and modulated protein B-cellular epitopes were predicted on the basis of a three-dimensional model of the protein and synthesized. The above-mentioned peptides were conjugated with the hemocyanin from the Megathura crenulata and with bovine serum albumin. The conjugates were used as antigens for immunization of the CBAxC57BL/6 mice. The antipeptide high-titer sera that reacted with the yeast-produced recombinant L1?C31 proteins were obtained. The peptides corresponding to the 49?C65 and 172?C189 sequences of the protein were found to have the maximum reactivity and type specificity. The isolated IgG-antibodies to these epitopes easily recognized L1-31, but did not recognize L1?C16. These antibodies were promising for the analysis of the L1-31 production in various expression systems.