The novel ADAMs-like microbial metalloendopeptidase |
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| Authors: | N P Balaban N L Rudakova A R Sabirova L R Valeeva M R Sharipova |
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| Institution: | 1. Department of Microbiology, Faculty of Biology and Soil Sciences, Kazan (Volga Region) State University, ul. Kremlevskaya 18, Kazan, 420008, Russia
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| Abstract: | Heterologous gene expression of the extracellular minor metalloendopeptidase of Bacillus pumilus 3?C19 (AN EU678894) in the protease-deficient B. pumilus 3?C19 strain has been studied. The enzyme fraction in the total pool of B. pumilus 3?C19 proteases is less than 8%. The enzyme was isolated from the culture liquid of the recombinant strain and purified to a homogeneous state; its primary structure was determined and its physicochemical properties were investigated. The secreted metalloendopeptidase of B. pumilus 3?C19 is the first prokaryotic homologue of the eukaryotic adamalysin/reprolysin protein family. |
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