Agrobacterium-Mediated Multiple Gene Transformation in Rice Using a Single Vector |
| |
| Authors: | Ming-Xia CAO Jian-Qiu HUANG Zhi-Ming WEI Quan-Hong YAO Chang-Zhao WAN Jia-An LU |
| |
| Institution: | State Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences;, Shanghai 200032, China; Agrobiotech Research Center, Shanghai Academy of Agricultural Sciences;, Shanghai 201106, China; Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences;, Shanghai 201106, China |
| |
| Abstract: | Abstract: The homodimeric hemoglobin gene ( VHb ), the trans -zeatin synthetase gene ( tzs ), the modified 5-enolpyruvylshikimate-3-phosphate synthase gene ( EPSPS ), a selectable marker gene ( hpt ), and a reporter gene ( gus ), as linked expression cassettes, were stacked into the T-DNA region of a binary vector and introduced simultaneously into immature embryos of the rice ( Oryza sativa L.) varieties Xiushui-11, Qiufeng, Youfeng, and Hanfeng by Agrobacterium tumefaciens . A total of 1 153 transgenic lines was obtained through selection for hygromycin B resistance. Approximately 90.2% of the transgenic lines harbored all the transgenes. Integration of multiple transgenes occurred at one to three genetic loci. Expression analysis revealed that the transgenes were coexpressed and inherited in a simple Mendelian fashion in transgenic plants and the frequency of coexpression was approximately 85%. On the basis of the cointegration and coexpression of the transgenes, most transgenic families were considered to be useful in a breeding program.
(Managing editor: Li-HuiZHAO) |
| |
| Keywords: | Agrobacterium tumefaciens linked expression cassettes multiple genes rice transformation |
| 本文献已被 维普 等数据库收录！ |
|
