Isolation and characterization of pLS 1 plasmid mutants with increased copy numbers |
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Authors: | Paloma Acebo,Marí a Teresa Alda,Manuel Espinosa,Gloria del Solar |
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Affiliation: | Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006 Madrid, Spain |
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Abstract: | Abstract Streptococcus pneumoniae genetic systems designed for isolation of plasmid mutants with copy-up phenotypes have been developed. The target plasmids have the pLS1 replicon, and two different strategies have been followed: (i) selection of clones exhibiting augmented resistance to antibiotics, or (ii) obligatory co-existence of incompatible plasmids. We have isolated 23 plasmid mutants exhibiting increased number of copies. All the mutations corresponded to four different alleles of the copG gene of plasmid pLS1. These strategies could be used with other plasmids. |
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Keywords: | Control of replication Plasmid copy number Selection of mutations |
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