Laccase activity from the fungus Trametes hirsuta using an air-lift bioreactor |
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Authors: | Rodríguez Couto S Rodríguez A Paterson R R M Lima N Teixeira J A |
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Institution: | Department of Chemical Engineering, University of Vigo, Vigo, Spain. susana.rodriguez@urv.net |
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Abstract: | AIM: To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB). METHODS AND RESULTS: Trametes hirsuta was grown in a 6-l ALB. A fed-batch strategy with glycerol as an addition resulted in maximum laccase activity of 19,400 U l(-1), which was the highest reported from the fungus. CONCLUSION: The ALB configuration with additional glycerol resulted in high laccase activities. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides useful information on how to produce high concentrations of laccase. |
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Keywords: | air-lift bioreactor carbon source glycerol laccase Trametes hirsuta |
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