酯酶同功酶在分析亚洲玉米螟抗药性中的应用

以聚丙烯酰胺凝胶电泳法对室内选育的亚洲玉米螟(Ostrinia furnacalis(Guenèe))抗杀灭菊酯和抗呋喃丹品系的酯酶同功酶进行分析,并与感性品系比较,差异十分明显,为鉴别害虫抗药性的遗传变异提供了有价值的依据,并从方法学的几个侧面进行探讨,为广泛研究害虫抗药性机理做一些基础工作。

APPLICATION OF ELECTROPHORETIC ESTERASE ISOZYME PATTERNS IN THE IDENTIFICATION OF INSECTICIDE-RESISTANT OF ASIAN CORN BORER

Polyacrylamide gel electrophoresis was used to determine esterase isozymes of three strains of Asian corn borer, Ostrinia furnacalis(Guenèe). Two strains were insecticide-resistant and one was susceptible. It is suggested that there were distinct differences in the zymograms of the esterase isozymes among the three strains, especially carboxylesterase. The number of cartoxylesterase bands of both resistant strains was more than that of the susceptible one, but there was no difference in thir cholinesterase. The zymograms were taken as a criterion to differentiate the resistant strain from the susceptible one. The method is suitable for studying resistance of some other agricultural insects and some aspects of methodology were discussed.