Negative-ion fast atom bombardment and electrospray ionization tandem mass spectrometry for characterization of sulfated and sialyl Lewis-type glycosphingolipids |
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Authors: | Tadashi II Yoko Ohashi Tomoya Ogawa Yoshitaka Nagai |
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Institution: | (1) Glycobiology Research, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, 351-01 Saitama, Japan;(2) Research and Development Laboratories, Soda Aromatic Co., Ltd, Noda-shi, 270-02 Chiba, Japan;(3) Laboratory of Synthetic Cellular Chemistry, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, 351-01 Saitama, Japan;(4) Faculty of Agriculture, University of Tokyo, Yayoi, Bunkyo-ku, 113 Tokyo, Japan |
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Abstract: | Structural characterization of sulfated and sialyl Lewis (Le)-type glycosphingolipids performed by fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry is described. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of acidic glycosphingolipids allowed identification of the sulfated or sialyl sugar, and provided information on the saccharide chain sequence. The negative-ion tandem FABMS of sulfated Le-type glycosphingolipids having the non-reducing end trisaccharide ion as the precursor can be used to differentiate the Lea- and LeX-type oligosaccharides. The ESI CID-MS/MS of multiple-charged ions provided even more detailed structural information, and some of the useful daughter ions appeared with higherm/z values than the precusor because of a lower charge-state. These methodologies can be applied to the structural analyses of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated and sialyl analogues.Abbreviations CID
collision-induced dissociation
- ESI
electrospray ionization
- FABMS
fast atom bombardment mass spectrometry
- Fuc
fucose
- Gal
galactose
- GlcNAc
N-acetylglucosamine
- Le
Lewis
- Lea
Lewisa
- LeX
LewisX
- MS/MS
mass spectrometry/mass spectrometry
- NeuAc
N-acetylneuraminic acid
- 3-SO4-Lea
3-sulfated Lea pentaosyl ceramide
- 3-SO4-LeX
3-sulfated LeX pentaosyl ceramide
- 2,3-SO4-LeX
2,3-disulfated LeX pentaosyl ceramide
- 3-S-Lea
3-sialyl Lea pentaosyl ceramide
- 3-S-Lex
3-sialyl LeX heptaosyl ceramide
- 3-S-LeX-LeX
3-sialyl-Lex-Lex octaosyl ceramide. |
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Keywords: | sulfated Lewisa sulfated LewisX sialyl Lewisa sialyl LewisX glycosphingolipid ESIMS FABMS CIDMS/MS |
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