Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardii : I. Loss and recovery of agglutinability of gametes treated with EDTA |
| |
Authors: | Tatsuaki Saito Yoshihiro Matsuda |
| |
Institution: | Laboratoire Associé no. 92 du CNRS, Département de Biologie Générale et Appliquée, Université Claude Bernard Lyon-I, 69622 Villeurbanne Cedex, France |
| |
Abstract: | The effect of EDTA on the mating-type-specific agglutinins located on the flagellar surfaces of Chlamydomonas reinhardii gametes was investigated. The mating-type minus (mt-) gametes lost their agglutinability without apparent loss of motility soon after addition of EDTA at low concentrations (1-2 mM). At the same time, the cells released into the medium agglutinins which can elicit agglutinative responses of mating-type plus (mt+) gametes specifically. When EDTA was neutralized with Mg2+ or removed by centrifugation, the mt- cells quickly replaced agglutinins by protein synthesis: the recovery process was sensitive to cycloheximide, but not to tunicamycin. The EDTA-treated mt+ gametes lost their agglutinins much more slowly than the mt- gametes. The replacement of mt+ agglutinins was inhibited by both cycloheximide and tunicamycin. |
| |
Keywords: | To whom offprint requests should be sent |
本文献已被 ScienceDirect PubMed 等数据库收录! |