NMR studies of the relationship between the changes of membrane lipids and the cisplatin-resistance of A549/DDP cells |
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Authors: | Zhenhua?Huang Yufeng?Tong Email author" target="_blank">Jinfeng?WangEmail author Email author" target="_blank">Youguo?HuangEmail author |
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Institution: | (1) National Laboratory of Biomacromolecules, Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China |
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Abstract: | Changes of membrane lipids in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP cells during the apoptotic process
induced by a clinical dose of cisplatin (30 μM) were detected by 1H and 31P-NMR spectroscopy and by membrane fluidity measurement. The apoptotic phenotypes of the two cell lines were monitored with
flow cytometry. The assays of apoptosis showed that significant apoptotic characteristics of the A549 cells were induced when
the cells were cultured for 24 hours after treatment with cisplatin, while no apoptotic characteristic could be detected for
the resistant A549/DDP cells even after 48 hours. The results of 1H-NMR spectroscopy demonstrated that the CH2/CH3 and Glu/Ct ratios of the membrane of A549 cells increased significantly, but those in A549/DDP cell membranes decreased.
In addition, the Chol/CH3 and Eth/Ct ratios decreased for the former but increased for the latter cells under the same conditions. 31P-NMR spectroscopy indicated levels of phosphomonoesters (PME) and ATP decreased in A549 but increased in A549/DDP cells after
being treated with cisplatin. These results were supported with the data obtained from 1H-NMR measurements. The results clearly indicated that components and properties of membrane phospholipids of the two cell
lines were significantly different during the apoptotic process when they were treated with a clinical dose of cisplatin.
Plasma membrane fluidity changes during cisplatin treatment as detected with the fluorescence probe TMA-DPH also indicate
marked difference between the two cell lines. We provided evidence that there are significant differences in plasma membrane
changes during treatment of cisplatin sensitive A549 and resistant A549/DDP cells. |
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