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In vivo Microdialysis of 5-Hydroxyindoleacetic Acid and Glutamic Acid in the Hamster Suprachiasmatic Nuclei |
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| Authors: | GLASS, J. DAVID HAUSER, URSULA E. RANDOLPH, WALTER W. REA, MICHEAL A. DE VRIES, MARTINUS J. |
| Affiliation: | Department of Biological Sciences, Kent State University Kent, Ohio 44242 U.S.A. F. School of Aerospace Medicine, Brooks A.F.B. San Antonio, Texas 78235 Department of Physiology and Physiological Physics, University of Leiden Leiden, The Netherlands |
| Abstract: | SYNOPSIS. The technique of in vivo brain microdialysis rapidlyis becoming a popular tool for research on the neurochemicalbasis of physiological and behavioral functions. The presentstudy describes the application of microdialysis to investigatethe endogenous release of 5-hydroxyindoleacetic acid (5-HIAA)and glutamic acid in the suprachiasmatic nuclei (SCN) of hamsters.There were apparent circadian patterns of release of both ofthese neurosecretions, with peak levels occurring during thedark phase. Pharmacological manipulations of serotonin releaseand reuptake, using tetrodotoxin and citalopram, respectively,provided evidence that the nocturnal increase in 5-HIAA reflectsan increase in serotonergic synaptic activity, rather than intraneuronalmetabolism of unreleased serotonin. These results illustratethe usefulness of the microdialysis technique for studies onthe neurochemistry of central pacemaker function. |
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