Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818) |
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| Authors: | Vanessa Siqueira Grazeffe, Lenita de Freitas Tallarico, Alessandro de S Pinheiro, Toshie Kawano, Miriam Fussae Suzuki, Kayo Okazaki, Carlos Alberto de Bragan a Pereira,Eliana Nakano |
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| Affiliation: | Vanessa Siqueira Grazeffe, Lenita de Freitas Tallarico, Alessandro de Sá Pinheiro, Toshie Kawano, Miriam Fussae Suzuki, Kayo Okazaki, Carlos Alberto de Bragança Pereira,Eliana Nakano, |
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| Abstract: | The single cell gel eletrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20 Gy of 60Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens. |
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| Keywords: | Biomphalaria Freshwater snail DNA damage Single cell gel/comet assay Ionizing radiation |
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