| 细胞色素P450酶与微生物药物创制北大核心CSCD |
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| 引用本文： | 李众,张伟,李盛英.细胞色素P450酶与微生物药物创制北大核心CSCD[J].微生物学报,2016,56(3):496-515. |
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| 作者姓名： | 李众 张伟 李盛英 |
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| 作者单位： | 中国科学院青岛生物能源与过程研究所, 山东省合成生物学省级重点实验室, 中国科学院生物燃料重点实验室, 山东 青岛 266101;中国科学院大学, 北京 100049,中国科学院青岛生物能源与过程研究所, 山东省合成生物学省级重点实验室, 中国科学院生物燃料重点实验室, 山东 青岛 266101,中国科学院青岛生物能源与过程研究所, 山东省合成生物学省级重点实验室, 中国科学院生物燃料重点实验室, 山东 青岛 266101 |
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| 基金项目： | 国家自然科学基金(31422002,31300075);山东省自然科学基金(JQ201407) |
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| 摘 要： | 细胞色素P450酶广泛存在于动植物和微生物体内,具有底物结构多样性和催化反应类型多样性,在天然产物生物合成中扮演重要作用。P450酶可在温和条件下高选择性地催化结构复杂有机化合物中惰性C-H键的氧化反应,具备化学催化剂难以比拟的优势,因此在微生物制药领域具有广阔的应用空间。本文综述了参与天然产物生物合成的P450酶近年来的研究进展;P450酶的酶工程改造、生物转化实践及其在微生物药物创制方面的应用现状;探讨了P450酶的工业应用瓶颈及其解决途径;并对P450酶未来的应用前景进行了展望。
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| 关 键 词： | 细胞色素P450酶 微生物制药 酶工程 生物转化 |
| 收稿时间： | 2015/9/11 0:00:00 |
| 修稿时间： | 2015/12/11 0:00:00 |
Cytochrome P450 enzymes and microbial drug development-A review |
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| Zhong Li,Wei Zhang and Shengying Li.Cytochrome P450 enzymes and microbial drug development-A review[J].Acta Microbiologica Sinica,2016,56(3):496-515. |
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| Authors: | Zhong Li Wei Zhang and Shengying Li |
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| Institution: | Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong Province, China;University of Chinese Academy of Sciences, Beijing 100049, China,Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong Province, China and Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong Province, China |
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| Abstract: | Cytochrome P450 enzymes broadly exist in animals, plants and microorganisms. This superfamily of monooxygenases holds the greatest diversity of substrate structures and catalytic reaction types among all enzymes. P450 enzymes play important roles in natural product biosynthesis. In particular, P450 enzymes are capable of catalyzing the regio-and stereospecific oxidation of non-activated C-H bonds in complex organic compounds under mild conditions, which overrides many chemical catalysts. This advantage thus warrants their great potential in microbial drug development. In this review, we introduce a variety of P450 enzymes involved in natural product biosynthesis; provide a brief overview on protein engineering, biotransformation and practical application of P450 enzymes; and discuss the limits, challenges and prospects of industrial application of P450 enzymes. |
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| Keywords: | cytochrome P450 enzymes microbial drug development protein engineering biotransformation |
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