Flow cytometric analysis of the recruitment of G0 cells in human epidermis in vivo following tape stripping |
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Authors: | J B M Boezeman F W Bauer R M de Grood |
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Institution: | Department of Dermatology, University of Nijmegen, Nijmegen, The Netherlands |
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Abstract: | Abstract. Tape stripping of human skin elicits a proliferative response of a synchronously-dividing group of cells. The progress of this cohort of cells has been monitored using two windows in the cell cycle, one located in mid-S phase and the other centred around G2+ M. The cellular DNA is measured with flow cytometry, the windows are defined by two ranges in the DNA histogram. The cohort can be described as the recruitment of cells from a pre-existing G0 compartment which consists of 76% of all proliferative cells. The duration of the S phase is calculated to be 10.2 hr and G2+ M phase 5.1 hr. The cell cycle time of 39 hr for normal human keratinocytes derived from these figures is in line with recent values obtained by different techniques. |
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