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A method for hydrolyzing and determining the amino acid compositions of picomole quantities of proteins in less than 3 hours |
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| Authors: | GL Lookhart BL Jones DB Cooper SB Hall |
| Institution: | 1. U.S. Grain Marketing Laboratory, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, 1515 College Ave., Manhattan, KS 66502, USA;2. Departments of Biochemistry and Grain Science and Industry, Kansas University, Manhattan, KS 66506, U.S.A. |
| Abstract: | A method is described for quantitatively hydrolyzing proteins in 45 min and for analyzing the hydrolysates by high-performance liquid chromatography in an additional 52 min. The α-amino acids were detected by the fluorescence of their o-phthaldialdehyde derivatives. Ten picomoles of each of the commonly occuring α-amino acids could be reliably determined. The method described yielded OPA-ethanethiolamino acid derivatives that were stable for h and the HPLC method produced a better separation than previously published methods. |
| Keywords: | protein hydrolysis amino acid analysis high-performance liquid chromatography orthopathaldiadehyde derivatives picomole sensitivity |
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