Involvement of an Inducible Cytochrome P-450 in Precocene II Oxidation by Streptomyces Griseus |
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Authors: | F. Sima Sariaslani Michael K. Trower Daniel A. Kunz |
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Affiliation: | a Central Research and Development Department, Experimental Station, Bldg 228/Rm, E.I. duPont de Nemours & Company Inc., Wilmington |
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Abstract: | Streptomyces griseus oxidizes the insecticide precocene II to its cis- and trans-dihydrodiols and 3-chromenol after growth on an enriched medium containing soybean flour. Oxidation of precocene II is dependent on the level of cytochrome P-450 in this organism. Extracts of cells grown on media lacking soybean flour were devoid of cytochrome P-450 and could not oxidize precocene II. In an in vitro reconstituted system containing NADPH, spinach ferredoxin reductase, spinach ferredoxin and ammonium sulfate fractions enriched in cytochrome P-450, precocene II was oxidized to its dihydrodiols. An aerial mycelium-negative variant of S. griseus (AMY mutant), that was unable to elicit cytochrome P-450 when grown on soybean flour-enriched medium, failed to oxidize precocene II. |
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Keywords: | Microbiol transformation precocene II monooxygenase cytochrome P-450 |
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