一株南极嗜冷杆菌产丙酮酸脱氢酶的性质研究及基因克隆

从南极普利兹湾深海沉积物中筛选到一株嗜冷杆菌7195。其16SrDNA序列分析表明谊菌株属于嗜冷杆菌属（Psychrobacter），从该菌的全基因组DNA中克隆到编码丙酮酸脱氢酶系E1（PDHcEI）的完整ORF，全长为2817bp，使用DNAMAN5．1对其全长ORF的PDHcEI基因进行分析，PDHcE1基因编码一个由939AA残基组成、分子量预计为100663Da的PDHcEI蛋白质，与Psychrobacter sp．273—4的PDHcE1有78．53％的相似性。

Characterization and Gene Cloning of the Pyruvate dehydrogenase E1 (PDHE1) from an Antarctic Psychrobacter Strain

A strain 7195, which produces Pyruvate dehydrogenase (E1), was isolated from the deep sea sediment of Prydz Bay, Antarctic. The morphology identification and 16S rDNA sequence analysis showed that it belonged to genus Psychrobacter. The Pyruvate dehydrogenase (E1) gene was cloned by PCR according to the primers. Nucleotide sequence analysis revealed an open reading frame (ORF) of 2817 bp encoding the PDHcE1, The amino acid sequence deduced from the nucleotide sequence of the PDHcE1 gene corresponded to a protein of 939 amino acid residues with a molecular weight of 100663 kDa. The PDHcE1 shows a high sequence, with homology of 78.53%, similar to the PDHcE1 gene from Psychrobacter sp. 273-4.