| iTRAQ技术及其在蛋白质组学中的应用 |
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| 引用本文： | 谢秀枝,王欣,刘丽华,董世雷,皮雄娥,刘伟.iTRAQ技术及其在蛋白质组学中的应用[J].中国生物化学与分子生物学报,2011,27(7):616-621. |
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| 作者姓名： | 谢秀枝 王欣 刘丽华 董世雷 皮雄娥 刘伟 |
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| 作者单位： | 内蒙古工业大学化工学院;浙江省农业科学院植物保护与微生物研究所;浙江省师范大学化学与生命学院; |
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| 基金项目： | 国际科技合作项目(No.2008DFA32080)~~ |
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| 摘 要： | 近年来随着蛋白质组学的迅速发展,其相应的方法学研究也取得了巨大的进步, 一系列新技术融入了蛋白质组学研究中,极大地促进了这门学科的发展.相对和绝对定量同位素标记(iTRAQ)技术与高度敏感性和准确性的串联质谱及多维液相色谱联用技术已成为蛋白质定性和定量研究的主要工具之一. 该技术可对复杂样本、细胞器、细 胞裂解液等样本进行相对和绝对定量研究，具有较好的定量效果、较高的重复性.由于其能够同时对多达8种样品进行标记分析,故在生命科学的各个领域得到了广泛的应用.本文对iTRAQ的原理、实验流程、优缺点及近几年的应用进展进行综述.
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| 关 键 词： | 相对和绝对定量同位素标记(iTRAQ)技术 蛋白质组学 串联质谱 多维液相色谱 生物标记物 |
| 收稿时间： | 2011-03-15 |
ITRAQ Technology and Its Application in Proteomics |
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| XIE Xiu-Zhi ,WANG Xin ,LIU Li-Hua ,DONG Shi-Lei ,PI Xiong-E,LIU Wei School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot ,China,Institute of Plant Protection , Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou ,School of Chemistry , Life Science,Zhejiang Normal University,Jinhua ,China.ITRAQ Technology and Its Application in Proteomics[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(7):616-621. |
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| Authors: | XIE Xiu-Zhi WANG Xin LIU Li-Hua DONG Shi-Lei PI Xiong-E LIU Wei School of Chemical Engineering Inner Mongolia University of Technology Hohhot China Institute of Plant Protection Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou School of Chemistry Life Science Zhejiang Normal University Jinhua China |
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| Institution: | XIE Xiu-Zhi 1),WANG Xin 2),LIU Li-Hua 1),DONG Shi-Lei 3),PI Xiong-E2),LIU Wei 2)(1) School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010051,China,2)Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,3)School of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China) |
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| Abstract: | In the past years,proteomics was developed quickly and its corresponding methodological research has also been made great progress,a series of new technologies blended in proteomics technology,particularly the new combination of multi-dimensional liquid chromatography and mass spectrometry(MS)-based technologies with protein fragmentation methods,enable us easily to identify the dynamics of proteins profiles in any complex biological process.Isobaric tags for relative and absolute quantification(iTRAQ) is o... |
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| Keywords: | isobaric tags for relative and absolute quantification(iTRAQ) proteomics tandem mass spectrometry multi-dimensional liquid chromatography biomarker |
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