| 泛耐药细菌NDM-1基困Taqman PCR快速检测方法的建立 |
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| 引用本文： | 杜昕颖,汪舟佳,王玉飞,邱少富,袁静,宋宏彬,孙岩松,陈泽良,黄留玉.泛耐药细菌NDM-1基困Taqman PCR快速检测方法的建立[J].中国生物工程杂志,2011,31(6):81-85. |
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| 作者姓名： | 杜昕颖 汪舟佳 王玉飞 邱少富 袁静 宋宏彬 孙岩松 陈泽良 黄留玉 |
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| 作者单位： | 军事医学科学院疾病预防控制所 北京 100071 |
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| 基金项目： | 传染病重大专项课题(2009ZX10004-205)资助项目 |
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| 摘 要： | 产NDM-1(New Delhi Metallo-β-lactamase 1,Ⅰ型新德里金属β-内酰胺酶)细菌是新近报道的一种泛耐药细菌,由于对绝大多数常用抗生素均耐药,又被称为超级细菌.目的:建立一种可快速检测泛耐药细菌NDM-1基因的Taqman探针实时荧光定量PCR法.方法:根据NDM-1基因序列,设计引物和Ta...
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| 关 键 词： | NDM-1 荧光定量PCR 检测 |
| 收稿时间： | 2011-01-11 |
| 修稿时间： | 2011-03-25 |
Development of a Rapid TaqMan Real-time PCR Assay for Detection of NDM-1 |
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| DU Xin-ying,WANG Zhou-jia,WANG Yu-fei,QIU Shao-fu,YUAN Jing,SONG Hong-bin,SUN Yan-song,CHEN Ze-liang,HUANG Liu-yu.Development of a Rapid TaqMan Real-time PCR Assay for Detection of NDM-1[J].China Biotechnology,2011,31(6):81-85. |
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| Authors: | DU Xin-ying WANG Zhou-jia WANG Yu-fei QIU Shao-fu YUAN Jing SONG Hong-bin SUN Yan-song CHEN Ze-liang HUANG Liu-yu |
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| Institution: | DU Xin-ying WANG Zhou-jia WANG Yu-fei QIU Shao-fu YUAN Jing SONG Hong-bin SUN Yan-song CHEN Ze-liang HUANG Liu-yu(Institute of Disease Control and Prevention,Academy of Military Medical Science,Beijing 100071,China) |
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| Abstract: | The gene for NDM-1(New Delhi metallo-beta-lactamase 1) is one member of a large gene family that encodes beta-lactamase enzymes.Bacteria that produce NDM-1 often referred to in the news media as superbugs because these bacterial are usually resistant to most antibiotics and infections caused by them are difficult to treat.NDM-1 gene was used for design of PCR primers and Taqman probe.Then a real-time quantitative PCR array for rapid detection of bacteria that produce NDM-1 was developed and its specifcity,s... |
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| Keywords: | NDM-1 Real time PCR Molecular detection |
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