Isolation and identification of two isomeric trihydroxy octadecenoic acids with prostaglandin E-like activity from onion bulbs () |
| |
Authors: | L. Üstünes M. Claeys G. Laekeman A.G. Herman A.J. Vlietinck A. Özer |
| |
Affiliation: | 1. Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium;2. Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey |
| |
Abstract: | Two fractions with prostaglandin E-like activity were isolated from onion () by using XAD-2 adsorption, silicic acid column chromatography and thin layer chromatography. The fractions were analyzed by gas chromatography/mass spectrometry and were characterized as isomeric mixtures of 9,10,13-trihydroxy-11-octadecenoic and 9,12,13-trihydroxy-10-octadecenoic acid, which are lipoxygenase metabolites of linoleic acid. Bio-assay, for which cascade superfusion was used and the rabbit coeliac and mesenteric arteries and the rat fundus strip were employed as assay organs, was utilized to monitor the bio-active profile throughout the isolation procedures. The activity of 1 μg of the pharmacologically active fractions T1 and T2 was found to be equivalent to that of respectively 1.33 and 0.63 ng of prostaglandin E2. |
| |
Keywords: | Send correspondence and reprint requests to M. Claeys. |
本文献已被 ScienceDirect 等数据库收录! |
|