Production of heterogeneous dimer lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli cells |
| |
| Authors: | Kamoda Shigehiro Terada Tamami Saburi Yoshimasa |
| |
| Institution: | University Forest in Hokkaido, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Hokkaido, Japan. kamo@uf.a.u-tokyo.ac.jp |
| |
| Abstract: | The lignostilbenedioxygenase isozyme I and III genes, lsdA and lsdB, were coexpressed within the same Escherichia coli cells. The lignostilbenedioxygenase isozymes produced were separated on QAE anion-exchange column chromatography. Three parts of active fractions corresponding to alphaalpha, alphabeta, and betabeta dimers were detected. The purified isozyme from second active fractions corresponded to the native heterogeneous isozyme II. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
