Immunohistochemical and immunocytochemical detection of SchS34 antigen in <Emphasis Type="Italic">Stachybotrys chartarum</Emphasis> spores
and spore impacted mouse lungs |
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Authors: | Thomas G Rand J David Miller |
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Institution: | (1) Department of Biology, Saint Mary’s University, Halifax, NS, Canada, B3H 3C3;(2) Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, ON, Canada, K1S 5B6 |
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Abstract: | The purpose of this study was to evaluate the distribution of a 34 kD antigen isolated from S. chartarum sensu lato in spores and in the mouse lung 48 h after intra-tracheal instillation of spores by immuno-histochemistry. This antigen was
localized in spore walls, primarily in the outer and inner wall layers and on the external wall surfaces with modest labelling
observed in cytoplasm. Immuno-histochemistry revealed that in spore impacted mouse lung, antigen was again observed in spore
walls, along the outside surface of the outer wall and in the intercellular space surrounding spores. In lung granulomas the
labelled antigen formed a diffusate, some 2–3× the size of the long axis of spores, with highest concentrations nearest to
spores. Collectively, these observations indicated that this protein not only displayed a high degree of specificity with
respect to its location in spores and wall fragments, but also that it slowly diffuses into surrounding lungs. |
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Keywords: | Stachybotrys chartarum Antigen Spores Mouse lung Immunohistochemistry Antigen processing |
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