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A microcalorimetric study of the effect of La3+ on mitochondria isolated from Star-Cross 288 chicken liver tissue cells
Authors:Yi Liu  Peng Liu  Fengjiao Deng  Zhong Chen  Cunxin Wang  Songsheng Qu
Affiliation:(1) Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China;(2) College of Life Sciences, Wuhan University, 430072 Wuhan, People’s Republic of China
Abstract:The thermogenic curves of the metabolism of mitochondria isolated from the liver of chicken Star-Cross 288 and the effect of La3+ on it were studied by using an LKB-2277 BioActivity Monitor, ampoule method, at 37°C. After isolation from the chicken liver tissue, mitochondria still have metabolic activity and can live for a long time by using the stored nutrients. From the thermogenic curves, we obtained the thermokinetic equations under different condition. The kinetic study shows that La3+ has changed the metabolism completely.
Keywords:Mitochondria  microcalorimetry  thermokinetics  thermochemistry  La3+
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