Induction of somatic embryogenesis in gum arabic tree [Acacia senegal (L.) Willd.] |
| |
| Authors: | Jitendra Singh Rathore Manoj K Rai N S Shekhawat |
| |
| Institution: | .Biotechnology Centre, Department of Botany, Jai Narain Vyas University, Jodhpur, 342033 Rajasthan India ;.Department of Botany and Biotechnology, L.M. College of Science and Technology, Jodhpur, 342001 Rajasthan India |
| |
| Abstract: | Factors affecting somatic embryogenesis from immature cotyledon of gum arabic tree Acacia senegal (L.) Willd.] were investigated. Induction of somatic embryogenesis was influenced by plant growth regulator concentrations and addition of amino acids in medium. Best induction of somatic embryogenesis was obtained on MS medium supplemented with 0.45 μM 2, 4-D, 2.32 μM Kin and 15 mM L-glutamine. L-glutamine plays a significant role in the maturation of somatic embryos and most of embryos attained maturity only on L-glutamine (15 mM) containing medium. Maximum percent (75.0 ± 2.5) germination of somatic embryos was recorded on medium containing 0.22 μM BAP. |
| |
| Keywords: | A senegal L-glutamine Plant growth regulators Somatic embryo induction |
|
|
