RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels
and use of different explant types |
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Authors: | Seemab Mukhtar Naseem Ahmad Md Imran Khan Mohammad Anis Ibrahim M Aref |
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Institution: | .Plant Biotechnology laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002 India ;.Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, PO Box 2460, Riyadh, 11451 Saudi Arabia |
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Abstract: | A comparative performance of two explants types (CN and Nodal) for their
efficiency to induce multiple shoot regeneration in Clitoria
ternatea has been carried out. Thidiazuron (TDZ) in different
concentrations (0.05–2.5 μM) was used as a supplement to the Murashige and Skoog’s
(MS) basal media. Explant type apart, two factors viz. concentration and exposure
duration to TDZ played an important role in affecting multiple shoot regeneration.
Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal
explants the highest rate of shoot formation was achieved on MS medium supplemented
with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the
regenerated shoots were subcultured on hormone free MS medium after 4 weeks of
exposure to TDZ. Among the two, cotyledonary node explants produced considerably
higher number of shoots at a comparatively lower concentration of TDZ than nodal
explants. The regenerated shoots rooted best on MS medium containing 1.0 μM
indole-3-butyric acid (IBA) and were successfully established in pots containing
garden soil with 88 % survival rate. All the regenerated plants showed normal
morphology and growth characteristics. |
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Keywords: | Thidiazuron Acclimatization Butterfly pea Cotyledonary node Node |
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