Binucleation and polyploidization patterns in developmental and regenerative rat liver growth |
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| Authors: | P. Gerlyng,A. Å byholm,T. Grotmol,B. Erikstein,H. S. Huitfeldt,T. Stokke,P. O. Seglen |
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| Affiliation: | Departments of Tissue Culture, Institute for Cancer Research the Norwegian Radium Hospital, Oslo, Norway;Departments of Pathology, Institute for Cancer Research the Norwegian Radium Hospital, Oslo, Norway;Departments of Biophysics, Institute for Cancer Research the Norwegian Radium Hospital, Oslo, Norway;Department of Environmental Health, the National Institute of Public Health, Oslo, Norway |
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| Abstract: | The hepatocellular binucleation rate, measured as the percentage of binuclear cells amongst newly formed bromodeoxyuridine-labelled and immunostained collage-nase-isolated rat hepatocytes, decreased from 12% to 4% between days 30 and 40 after birth, rose to 20% between days 50 and 60, and then declined again to the adult rate of about 10% at day 80. During regenerative growth following a two-thirds partial hepatectomy, the rate of binucleation declined to about 3%, causing the fraction of binuclear cells to fall from 27% (before hepactectomy) to 5% (at 45 h after hepactectomy) as pre-existing binuclear cells replicated and formed mononuclear daughter cells. Essentially all (97%) hepatocytes replicated at least once, starting their DNA synthesis at around 13 h and reaching a peak at 30 h, irrespective of ploidy and nuclearity. At later time points, the diploid hepatocytes had a higher labelling index than the polyploid cells, suggesting a greater tendency to go through several cell cycles. |
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