Regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using a novel 1,6-α-
-mannosidase from Aspergillus phoenicis |
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Authors: | Vasileios I Athanasopoulos Keshavan Niranjan Robert A Rastall |
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Institution: | School of Food Biosciences, The University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6 AP, UK |
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Abstract: | A novel 1,6-α-
-mannosidase was produced by Aspergillus phoenicis grown on a commercial manno-oligosaccharide preparation in liquid culture. The enzyme hydrolysed only α-
-Manp-(1→6)-
-Manp and did not act on α-
-Manp-(1→2)-
-Manp, or α-
-Manp-(1→3)-
-Manp. The 1,6-α-
-mannosidase was used for synthesis of manno-oligosaccharides by reverse hydrolysis reaction. The highest yields, expressed as percentages (w/w) of total sugar, were 21% mannobiose and 5% mannotriose, and they were obtained with 45% (w/w) initial mannose concentration at pH 4.5 after 12 days incubation at 55 °C. The disaccharide and trisaccharide products were separated and their structures determined by methylation analysis. Only 1–6 linkages were found in both of them. |
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Keywords: | Mannobiose Mannotriose Oligosaccharide synthesis 1 6-α -
-Mannosidase Aspergillus phoenicis |
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