Micropropagation of Guazuma crinita mart. by root and petiole culture |
| |
| Authors: | Emilio Maruyama Katsuaki Ishii Isao Kinoshita Kihachiro Ohba Akira Saito |
| |
| Affiliation: | (1) Institute of Agriculture and Forestry, University of Tsukuba, Tennodai 1-1-1, 305 Tsukuba, Ibaraki, Japan;(2) Bio-Resources Technology Division, Forestry and Forest Products Research Institute, Isukuba Norinkenkyu Danchi-Nai, P. O. Box 16, 305 Ibaraki, Japan;(3) Faculty of Agriculture, Kyushu University, 812 Fukuoka, Japan |
| |
| Abstract: | Summary Shoot organogenesis of Guazuma crinita Mart. from root and petiole explants was obtained via adventitious bud formation. Root segments and petiole explants excised from in vitro generated plantlets were cultured on woody plant medium (WPM) supplemented with [trans-6-(4-hydroxy-3-methylbut-2- enyl)aminopurine] (zeatin) or with [6-benzyladenine] (BA). After 45 d of culturing, clumps of green bulbous structures containing small adventitious buds (clusters) were generated in all explants cultured with 10 μM zeatin under a photon flux density of 65 μmol m−2 s−1. For subsequent shoot differentiation, clusters were transferred onto medium containing 1 μM zeatin. After 60 d of culturing, 30% of clusters generated from petiole explants developed into plants. The regenerated plantlets were successfully acclimatized and all survived and grew well. No morphological abnormalities were observed. |
| |
| Keywords: | Amazonian trees Bolaina blanca micropropagation organogenesis Sterculiaceae tissue culture of tropical trees |
| 本文献已被 SpringerLink 等数据库收录！ |
|
