Mutants of Volvox carteri affecting nitrogen assimilation |
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| Authors: | Robert J Huskey Clay F Semenkovich Barbara E Griffin Patricia O Cecil Ann M Callahan Kenneth V Chace and David L Kirk |
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| Institution: | (1) Department of Biology, University of Virginia, 22901 Charlottesville, Virginia, USA;(2) Present address: School of Medicine, Washington University, St. Louis, MO;(3) Present address: Department of Physiology, University of Virginia Medical Center, Charlottesville, VA;(4) Present address: Department of Biology, Washington University, St. Louis, MO |
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| Abstract: | Summary Three genes of Volvox carteri f. nagariensis have been identified which affect nitrogen assimilation. Mutants in two unlinked genes, nitA and nitC, were isolated as chlorate resistant and they exhibit no measureable nitrate reductase activity. The mutant in the nitB gene which is linked to nitA has slightly reduced levels of nitrate reductase activity and grows poorly on the nitrate concentration in standard medium but grows normally if the level of nitrate is increased. All of the mutants utilize ammonia, urea or nitrite for growth.Deseased |
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