Change in subcellular localization of overexpressed vaccine peptide in rice endosperm cell that is caused by suppression of endogenous seed storage proteins |
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Authors: | Mehrnaz Entesari Yuhya Wakasa Bahram Maleki Zanjani Fumio Takaiwa |
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Institution: | 1.Division of Biotechnology, Plant Molecular Farming Unit, Institute of Agrobiological Sciences,National Agriculture and Food Research Organization,Tsukuba,Japan;2.Department of Agronomy & Plant Breeding, Faculty of Agriculture,University of Zanjan,Zanjan,Iran |
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Abstract: | Rice-based peptide vaccine based on T cell epitopes acts as an ideal oral tolerogen for the treatment of type 1 allergic diseases. To improve production yields of oral tolerogen against Japanese cedar pollen allergy, hybrid peptide comprising seven predominant human T cell epitopes (7Crp) derived from Japanese cedar pollen allergens, Cry j 1 and Cry j 2, was produced in transgenic rice seed by expression of its codon optimized gene under the control of the endosperm-specific 26 kD globulin (Glb-1) promoter containing its signal peptide and the simultaneous suppression of endogenous seed storage proteins (SSPs) by RNA interference. Accumulation level of 7Crp peptide produced as a secretory protein was remarkably enhanced by suppression of both the 13–14 kDa prolamins and GluA and GluB glutelins as compared to those under suppression of either of them or in wild type rice. When these SSPs were down-regulated, the 7Crp peptide was observed to be localized in ER lumen as well as ER derived PBs (PB-Is). Especially, accumulation as self-aggregates in ER lumen increased by reduction of the endogenous 13–14 kDa prolamins. It is interesting to note that the absence of C terminal KDEL ER retention signal from the 7Crp peptide resulted in higher level accumulation (116 µg/grain) than that containing the KDEL. |
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