NurA,a novel 5'-3' nuclease gene linked to rad50 and mre11 homologs of thermophilic Archaea |
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Authors: | Constantinesco Florence Forterre Patrick Elie Christiane |
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Affiliation: | Institut de Génétique et Microbiologie, UMR CNRS no 8621, Batiment 409, Université Paris-Sud, 91405 Orsay cedex, France. |
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Abstract: | We isolated and characterized a new nuclease (NurA) exhibiting both single-stranded endonuclease activity and 5′–3′ exonuclease activity on single-stranded and double-stranded DNA from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Nuclease homologs are detected in all thermophilic archaea and, in most species, the nurA gene is organized in an operon-like structure with rad50 and mre11 archaeal homologs. This nuclease might thus act in concert with Rad50 and Mre11 proteins in archaeal recombination/repair. To our knowledge, this is the first report of a 5′–3′ nuclease potentially associated with Rad50 and Mre11-like proteins that may lead to the processing of double-stranded breaks in 3′ single-stranded tails. |
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