An improved culture system for secondary palatal elevation |
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| Authors: | Cindy Arey Lewis Larry Thibault Robert M. Pratt Linda L. Brinkley |
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| Affiliation: | (1) Laboratory of Developmental Biology, National Institute of Dental Research, 20205 Bethesda, Maryland;(2) Present address: Scripps Institution of Oceanography, Marine Biology Research Division A-002, University of California at San Diego, 92093 La Jolla, California;(3) Present address: Biomedical Engineering and Instrumentation Branch, Division of Research Services, National Institutes of Health, 20014 Bethesda, Maryland;(4) Present address: Craniofacial Development Section, Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Building 30, Room 405, Bethesda, Maryland;(5) Department of Anatomy, Medical School, University of Michigan, 48109 Ann Arbor, Michigan |
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| Abstract: | Summary An organ culture system devised for studying the development of the secondary palate was modified so that it retained high partial pressures of oxygen and supported total anterior and posterior palatal elevation. The cultured tissues appeared healthy as judged by histological examination. Medium was continuously recirculated through the culture system in which Day 13 embryonic mouse heads, with the brain and tongue removed, were totally submerged and suspended. The medium was constantly gassed via hollow fiber devices. A motor-driven stirrer, run at a low rate, agitated the medium so that the boundary layer surrounding the tissue was dispersed. Embryonic mouse heads were cultured in each of four media: Eagle's basal medium, Ham's F-12 medium, Fitton-Jackson's modified BGJb medium, and Waymouth's MB 752/1 medium. Elevation of the palate in both anterior and posterior regions with excellent tissue viability was achieved in all heads grown in BGJb medium. This work was supported by NIH Post-doctoral Fellowship 2F32 DE05038-03 to C. A. L. |
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| Keywords: | organ culture palate embryonic mouse |
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