角质细胞生长因子-2与核糖体蛋白L22相互作用的发现及在哺乳动物细胞中的验证

通过聚合酶链式反应从人胎肝cDNA文库中钓取KGF-2 cDNA，构建诱饵蛋白载体pAS2-1-KGF-2并对其自身转录激活活性进行鉴定，利用酵母双杂交系统筛选人胎肝cDNA文库，挑选双阳性克隆.DNA序列分析和同源检索显示,所获侯选蛋白为人核糖体蛋白L22(RPL22).将KGF-2和侯选蛋白分别克隆至哺乳动物细胞双杂交的BD、AD质粒中，共同转染COS-7细胞，通过CAT分析验证了KGF-2和侯选蛋白之间的相互作用.为阐明KGF-2作用的分子机制提供有益线索.

Interaction Between Human Ribosomal Protein L22 and Keratinocyte Growth Factor-2 and Its Identification in Mammalian Cell Line

The cDNA encoding keratinocyte growth factor-2 was isolated from human fetal liver cDNA library and subsequently cloned into the bait protein plasmid pAS2-1.The positive clone was obtained from human fetal liver cDNA library screened by yeast two-hybrid system. After sequence analysis and homology comparison, the candidate protein was identified as human ribosomal protein L22.KGF-2 and the candidate proteins were separately cloned into BD and AD plasmid of mammalian two-hybrid assay and cotransfected into COS-7 cell line. The interaction between KGF-2 and the candidate protein was identified by CAT assay.