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Thermophilic methanogenesis from sugar beet pulp by a defined mixed bacterial culture
Authors:B. Ollivier  N. Smiti  J. L. Garcia
Affiliation:(1) Laboratoire de Microbiologie ORSTOM, Université de Provence, 3, Place Victor-Hugo, 13331 Marseille cédex 3, France
Abstract:Summary Thermophilic degradation of sugar beet pulp was studied in batch cultures at 55°C by different associations of bacteria, includingClostridiumthermocellum,Methanobacterium sp. andMethanosarcina MP.C. thermocellum produced acetate, succinate, methanol, ethanol, H2 and CO2. The coculture ofC. thermocellum andMethanobacterium sp. produced trace amounts of ethanol and succinate; acetate concentration was about three times higher than in theC. thermocellum monoculture. The association of this coculture withMethanosarcina MP produced 5.5 mmol CH4/g dry weight sugar beet pulp.
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