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Thermophilic methanogenesis from sugar beet pulp by a defined mixed bacterial culture
Authors:B Ollivier  N Smiti  J L Garcia
Institution:(1) Laboratoire de Microbiologie ORSTOM, Université de Provence, 3, Place Victor-Hugo, 13331 Marseille cédex 3, France
Abstract:Summary Thermophilic degradation of sugar beet pulp was studied in batch cultures at 55°C by different associations of bacteria, includingClostridium thermocellum,Methanobacterium sp. andMethanosarcina MP.C. thermocellum produced acetate, succinate, methanol, ethanol, H2 and CO2. The coculture ofC. thermocellum andMethanobacterium sp. produced trace amounts of ethanol and succinate; acetate concentration was about three times higher than in theC. thermocellum monoculture. The association of this coculture withMethanosarcina MP produced 5.5 mmol CH4/g dry weight sugar beet pulp.
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