甲真菌病患者甲微生物群分析

目的研究甲真菌病患者甲微生物群的构成,为进一步阐明甲真菌病的发病机制提供线索。方法本研究共纳入47例甲真菌病患者及7例健康志愿者。取患者病甲的甲屑进行真菌镜检及培养鉴定;提取患者病甲、患者对侧健甲及健康人甲的DNA,对真菌rDNA ITS区及细菌16S rDNA V3-V4区PCR扩增,分析微生物群构成,并进行α多样性、β多样性、Simper分析及Spearman相关性分析。结果甲真菌病患者病甲的微生物群构成与对侧健甲及健康人甲存在差异。患者病甲的真菌菌群的丰富度高于对侧健甲。患者对侧健甲细菌菌群的多样性高于病甲及健康人甲,且患者对侧健甲细菌菌群的构成与患甲和健康人甲均有部分重叠。结论患者对侧健甲的细菌菌群构成具有由健康人甲向患者病甲转变的趋势。且在患者病甲的微生物群中,某些真菌和细菌菌属可能具有相关性。

Microbiome analysis of nails in patients with onychomycosis

Objective To analyze the structure of microbial community of onychomycosis and provide evidence for further elucidating the pathogenesis of onychomycosis.Methods A total of 47 patients with onychomycosis and 7 healthy volunteers were enrolled.Specimens from the nail scraps of the patients were examined by microscopy and culture.DNA was extracted from the diseased nail scraps of patients,healthy nail scraps of patients,and healthy nail scraps of volunteers.ITS sequence analysis of fungal rDNA and V3-V4 sequence analysis of bacterial 16S rDNA were performed by PCR amplification to analyze the microbiome.Andαdiversity,βdiversity,Simper analysis,and Spearman related analysis were also performed.ResultsThe structures of microbial community were different in diseased nails,healthy nails of patients with onychomycosis,and nails of healthy volunteers.The richness of fungal community in diseased nails was higher than that of healthy nails of patients with onychomycosis.The bacterial diversity in healthy nails of patients was the highest.The structure of bacterial community in self-control group was partially overlapped with that in patient group and healthy control group.Conclusions The structure of bacterial community in healthy nails of patients with onychomycosis has a tendency changing from that of a healthy nail to a diseased nail.Some fungi and bacteria may interact with each other.