Binding of sterols affects membrane functioning and sphingolipid composition in wheat roots |
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Authors: | Yu N Valitova E R Kotlova A V Novikov A L Shavarda K A Artemenko R A Zubarev F V Minibayeva |
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Institution: | 1.Institute of Biochemistry and Biophysics,Russian Academy of Sciences,Kazan,Russia;2.Komarov Botanical Institute,Russian Academy of Sciences,St. Petersburg,Russia;3.Institute of Analytical Instrument Engineering,Russian Academy of Sciences,St. Petersburg,Russia;4.Uppsala University,Uppsala,Sweden |
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Abstract: | The present work was devoted to the exploration of the role of sterols in the functioning of membranes in root cells. Membrane
characteristics and composition of the membrane lipids in the roots of wheat (Triticum aestivum L.) seedlings treated with exogenous cholesterol and antibiotic nystatin, which specifically binds with endogenous sterols,
were analyzed. Cholesterol caused a fall of membrane potential, acidification of the incubation medium, decrease in potassium
leakage of roots, and increase in the level of exogenous superoxide radical. Similarly to cholesterol, the application of
nystatin also induced the depolarization of the plasma membrane, but in contrast with cholesterol it was accompanied by alkalinization
of the incubation medium and decrease in the level of exogenous superoxide radical. Analysis of membrane lipids showed that
following nystatin treatment the total sterol content in roots did not change, while the level of complex sphingolipids represented
mainly by glycoceramides became higher. Using mass spectrometry with electrospray ionization (+ESI-MS) for the analysis of
the glycoceramide composition, we showed that nystatin induced changes in the ratios of molecular species of glycoceramides.
It was suggested that the modification of the sterol component of plasma membrane could influence membrane functioning by
changing the sphingolipid composition of lipid rafts. |
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