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Chromosomal localization and preliminary characterization of the human gene encoding insulin-like growth factor II |
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| Authors: | P de Pagter-Holthuizen J W M Höppener M Jansen A H M Geurts van Kessel G J B van Ommen J S Sussenbach |
| Institution: | (1) Laboratory for Physiological Chemistry, State University of Utrecht, Vondellaan 24A, NL 3521 GG Utrecht, The Netherlands;(2) Institute of Molecular Biology, State University of Utrecht, Utrecht, The Netherlands;(3) Department of Pediatrics, State University of Utrecht, Utrecht, The Netherlands;(4) Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands;(5) Department of Human Genetics, State University of Leiden, Leiden, The Netherlands |
| Abstract: | Summary A cDNA probe corresponding to mRNA encoding a closely related variant of human insulin-like growth factor II (IGF-II) was used for the chromosomal assignment of the IGF-II gene. Southern blot hybridization analysis of DNA from human-Chinese hamster somatic cell hybrids showed that the human IGF-II gene is located on chromosome 11. Using the same IGF-II variant probe, a cosmid was isolated which contains the human IGF-II gene. Restriction enzyme analysis revealed that the gene encoding IGF-II has a discontinuous structure and contains at least four exons. |
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