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Application of Genetic Bit Analysis (GBATM) for allelic selection in plant breeding |
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| Authors: | Alcala Josefina Giovannoni James J Pike Leonard M Reddy Avutu S |
| Institution: | (1) Vegetable Improvement Center, Centeq Research Plaza, Suite 120, 1500 Research Parkway, Texas A&M University Research Park, College Station, TX 77843-2119, USA;(2) Department of Horticultural Sciences and Crop Biotechnology Center, Texas A&M University, College Station, TX 77843-2133, USA;(3) Laboratory for Crop Genome Analysis, Crop Biotechnology Center and Dept. of Soil and Crop Sciences, Texas Agricultural Experiment Station, Texas A&M University System, College Station, TX 77843-2123, USA |
| Abstract: | Genetic Bit Analysis (GBA) is a relatively new technique developed to score single-nucleotide polymorphisms among alleles. It is currently being used as a genetic diagnostic tool in human paternity tests as well as in pedigree analysis of farm animals. GBA relies on the polymerase chain reaction and enzyme-linked colorimetry to distinguish differences among genotypes. The identification of a single-nucleotide polymorphism which distinguished the plastome of cytoplasmic male-sterile onion varieties from the plastome of fertile lines provided a model system for testing the utility of GBA in plants. Our results demonstrate that GBA permits rapid and accurate allele determination in onion breeding lines, resulting in accurate prediction of sterility at the seedling stage. The use of 96-well microtiter plates and automated liquid-handlers allowed semi-automation of Genetic Bit Analysis. |
| Keywords: | Allium cepa cytoplasmic male sterility DNA diagnostics Genetic Bit Analysis onion single-nucleotide polymorphism |
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