A nuclear factor requires Zn2+ to bind a regulatory MRE element of the mouse gene encoding metallothionein-1 |
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Authors: | C Séguin |
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Affiliation: | Centre de Recherche en Cancérologie de l'Université Laval, L'H?tel-Dieu de Québec, Canada. |
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Abstract: | The metal ion requirement of nuclear proteins for binding to the metal regulatory element d(MREd) of the mouse gene encoding metallothionein-1 was investigated using an in vitro exonuclease III footprinting assay. The specific DNA-binding activity of the factor was inactivated by the chelating agents, EDTA and 1,10-phenanthroline. Binding activity was restored by Zn2+, but not by Cd2+. These results show that Zn2+ ions are a required component for specific in vitro DNA binding of the MREd-binding protein. |
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