Phylogenetic analyses within three sections of the genus Vicia |
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Authors: | Tomasz Sakowicz Tomasz Cieślikowski |
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Institution: | (1) Department of Cytogenetics and Plant Molecular Biology, University of Łódź, 90-237 Łódź, Banacha 12/16, Poland;(2) Centre for Medical Biology Polish Academy of Sciences, 93-232 Łódź, Lodowa 106, Poland |
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Abstract: | The averaged genomic similarities based on multilocus randomly amplified polymorphic DNA (RAPD) were calculated for eight
species representing three sections of the genus Vicia: faba, bithynica and narbonensis. The frequency of appearance of the sequences corresponding to 25 decamers selected at random
from genomes of different Fabace species was checked, and a high correlation with the frequency observed for Vicia allowed us to assume their similar weight in typing Vicia species. The RAPD-based similarity coefficients compared with those related to whole genome hybridization with barley rDNA
and those based on restriction fragment length polymorphism (RFLP) revealed similar interspecies relationships. The averaged
RAPD-based similarity coefficient (Pearson’s) was 0.68 for all the species, and was sectionspecific: 0.43 (bithynica), 0.50
(faba) and 0.73 (narbonensis). The averaged similarity coefficient for V. serratifolia (0.63) placed it apart from the rest (0.75) of its section. The results correspond to the interspecies relationships built
upon non-genetic data. The averaged similarity coefficient for particular RAPD was related to the presence and type of tandemly
repeated motif in a primer: 0.7–0.8 for heterodimers (GC, AG, CA, GT, CT), 0.5–0.6 for homodimers (CC, GG) and 0.6 for no
repeat, indicating the sensitivity of diversity range to the type of target sequences. |
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Keywords: | Vicia Average similarity Multilocus diversity RAPD |
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