Construction of artificial epithelial tissues prepared from human normal fibroblasts and C9 cervical epithelial cancer cells carrying human papillomavirus type 18 genes |
| |
Authors: | Eun Kyung Yang Seung Won Jin Byung Tae Kang Ik Hwan Kim Jung Keug Park Sang Sook Lee Jin Woo Kim Sue Nie Park |
| |
Institution: | 1. Department of Chemical Engineering, Dongguk University, 100-715, Seoul, Korea 2. Virus and Oncology Research Unit, Molecular and Cellular Biology Research Division, Korea Research Institute of Bioscience & Biotechnology, KIST, 305-600, Taejon, Korea 3. Graduate School of Biotechnology, Korea University, 136-701, Seoul, Korea 4. Department of Pathology School of Medicine, Keimyung University, 700-712, Taegu, Korea 5. Department of Ob/Gyn., Medical College, Catholic University, 137-701, Seoul, Korea
|
| |
Abstract: | One cervical cancer cell line, C9, carrying human papillomavirus type 18 (HPV18) genes that is one of the major etiologic
oncoviruses for cervical cancer was characterized. This cell line was further characterized for its capacity related to the
epithelial cell proliferation, stratification and differentiation in reconstituted artificial epithelial tissue. Thein vitro construction of three dimensional artificial cervical epithelial tissue has been engineered using C9 epithelial cancer cells,
human foreskin fibroblasts and a matrix made of type I collagen by organotypic culture of epithelial cells. The morphology
of paraffin embedded artificial tissue was examined by histochemical staining. The artificial epithelial tissues were well
developed having multilayer. However, the tissue morphology was similar to the cervical tissue having displasia induced by
HPV infection. The characteristics of the artificial tissues were examined by determining the expression of specific marker
proteins. In the C9 derived artificial tissues, the expression of EGF receptor, an epithelial proliferation marker proteins
for stratum basale was observed up to the stratum spinosum. Another epithelial proliferation marker for stratum spinosum,
cytokeratins 5/6/18, were observed well over the stratum spinosum. For the differentiation markers, the expression of involucrin
and filaggrin were observed while the terminal differentiation marker, cytokeratins 10/13 were not detected at all. Therefore
the reconstituted artificial epithelial tissues expressed the same types of differentiation marker proteins that are expressed
in normal human cervical epithelial tissues but lacked the final differentiation capacity representing characteristics of
C9 cell line as a cancer tissue derived cell line. Expression of HPV18 E6 oncoprotein was also observed in this artificial
cervical epithelial tissue though the intensity of the staining was weak. Thus this artificial cervical epithelial tissue
though the intensity of the staining was weak. Thus this artificial epithelial tissue could be used as a useful model system
to examine the relationship between HPV-induced cervical oncogenesis and epithelial cell differentiation. |
| |
Keywords: | artificial epithelial tissue differentiation tissue engineering human papillomavirus (HPV) oncogenesis |
本文献已被 SpringerLink 等数据库收录! |
|