X-ray small-angle scattering study of mononucleosomes and of the close packing of nucleosomes in polynucleosomes

The radius of gyration of mononucleosomes determined by X-ray small-angle scattering is 4.35 nm. The maximum dimension determined from the distance distribution function and the volume amount to 12.9 nm and 370 nm³, respectively. For a particular fraction of polynucleosomes a mean radius of gyration 16 nm, a maximum dimension 65 nm, and a mean volume 25,240 nm³ is obtained.The shape is approximated by an elongated cylinder having a diameter of 28 nm. A polynucleosome is built up from 69 nucleosomes, on the average. The distance of neighbouring nucleosomes in the polynucleosome amounts to 5.2 nm. Moreover, this distance shows that the nucleosomes in the polynucleosome are very closely packed.