Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant |
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Authors: | Yokoyama Kazuaki Nakagawa Miyuki Satoh Masayuki Saitoh Shigeaki Dohmae Naoshi Harada Ayako Satoh Noriko Karasawa Ken Takio Koji Yanagida Mitsuhiro Inoue Keizo |
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Institution: | Faculty of Pharmaceutical Sciences, Teikyo University, Suarashi, Sagamiko-machi, Sagamihara-shi, Kanagawa, Japan. yokoyama@pharm.teikyo-u.ac.jp |
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Abstract: | The fission yeast lsd1/fas2 strain carries a temperature-sensitive mutation of the fatty-acid-synthase alpha-subunit, exhibiting an aberrant mitosis lsd phenotype, with accumulation of very-long-chain fatty-acid-containing phospholipid (VLCFA-PL). A novel 90-kDa protein, Lsd90 (SPBC16E9.16c), was found to be newly expressed in small particle-like structures in lsd1/fas2 cells under restrictive conditions. Two mismatches leading to a double frame shift were found between the sequences of the lsd90(+) gene registered in the genomic database and the sequences determined experimentally at the amino acid, cDNA and genomic DNA levels. Unexpectedly, overexpression and disruption of the lsd90(+) gene in either lsd1/fas2 or wild-type cells did not affect either cell growth or expression of the lsd phenotype. The amounts of VLCFA-PL that accumulated in lsd90-overexpressing lsd1/fas2 cells were significantly lower than those in lsd1/fas2 cells, suggesting the involvement of Lsd90 in the metabolism of VLCFA-PL. |
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