Analysis of the frequency and distribution of sister chromatid exchanges in cultured human lymphocytes |
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Authors: | Crossen Peter E Drets Maximo E Arrighi Frances E Johnston Dennis A |
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Institution: | (1) Cytogenetics Unit, Christchurch Hospital, Christchurch, New Zealand;(2) Division de Citogenetica Humana, Instituto de Investigacion de Ciencias Biologicas, Montevideo, Uruguay;(3) Department of Biology and Department of Biomathematics, The University of Texas System Cancer Center M.D. Anderson Hospital and Tumor Institute, 77030 Houston, Texas, USA |
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Abstract: | Summary Lymphocytes from 20 normal subjects (11 male and 9 female) were examined for the frequency and location of sister chromatid exchanges (SCE) by the BrdU—Giemsa method. The mean frequency of SCE was 6.37 with little significant variation. One subject had a high number of exchanges in chromosome 1 while the remainder showed a random distribution of exchanges between chromosomes. The frequency of exchanges generally increased with chromosome length. However, chromosome 1, 2 and the B group had more exchanges than expected while the E, F and G groups had less than expected. The distribution of exchanges in chromosomes 1, 2 and the B group was non-random with a concentration of exchanges below the centromere and to a lesser extent on the distal portion of the long arm. The majority of exchanges appeared to occur at the junction between the dark and light G bands. It is suggested that the concentration of exchanges may reflect differences in BrdU incorporation along the length of the chromosome. |
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