Chitinolytic enzymes from the newly isolated <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> SBK1: study of the mosquitocidal activity |
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Authors: | Suman Kumar Halder Chiranjit Maity Arijit Jana Bikas Ranjan Pati Keshab Chandra Mondal |
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Institution: | (1) Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India; |
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Abstract: | Aeromonas hydrophila SBK1 (GenBank accession no. HM802878.1), a potent chitinolytic bacterium, was isolated from a pool of 30 chitinolytic isolates.
The isolate showed higher chitinolytic activity in respect to clear zone to colony size ratio of 2.15. Maximum production
of chitinolytic enzymes, viz., β-N-acetyl-glucosaminidase and chitinase (specific activity 655.3 and 71.6 U mg−1, respectively) by A. hydrophila SBK1 was observed in the synthetic media, containing (w/v)-colloidal chitin, 4.0%; peptone, 0.3%; phosphate, 0.3% (0.15%
of each KH2PO4 and K2HPO4); NaCl, 0.25%; MgSO4, 0.05%; KCl, 0.05%; pH 7.0 and at 35°C after 72 h of incubation. Both carbon-to-nitrogen (C/N) and carbon-to-phosphate (C/P)
ratio of 13.33 were found optimum for chitinase production. Enzyme productivity increased about twofold in optimized culture
condition in respect to its un-optimized state. The crude enzyme showed optimum activity against Culex
quinquefasciatus larvae in native water at pH 7.0 and 35°C (LD50 0.60 U ml−1 at 48 h). Therefore, the studied chitinases can be used as an effective mosquitocidal agent. |
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